Journal: BioMed Research International

Article Title: Next-Generation Sequencing Panel Analysis of Clinically Relevant Mutations in Circulating Cell-Free DNA from Patients with Gestational Trophoblastic Neoplasia: A Pilot Study

doi: 10.1155/2020/1314967

Figure Lengend Snippet: Details of overlapping mutations.

Article Snippet: Reagents included antiglyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (cat. no. KC-5G4; Aksomics, Shanghai, China), antibone morphogenetic protein receptor type 1A (BMPR1A) antibody (cat. no. 12702-1-AP; Proteintech, IL, USA), antimitogen-activated protein kinase kinase kinase 1 (MAP3K1) antibody (cat. no. 19970-1-AP; Proteintech, IL, USA), Magnetic Serum/Plasma DNA Maxi Kit (Tiangen, Beijing, China), TRIzol reagent (Invitrogen, CA, USA), PrimeScript RT kit, and SYBR Premix Ex Taq II (Takara, Shiga, Japan).

Techniques:

Journal: BioMed Research International

Article Title: Next-Generation Sequencing Panel Analysis of Clinically Relevant Mutations in Circulating Cell-Free DNA from Patients with Gestational Trophoblastic Neoplasia: A Pilot Study

doi: 10.1155/2020/1314967

Figure Lengend Snippet: Protein expression of BMPR1A and MAP3K1 in three cell lines. (a) Western blotting gel image for BMPR1A and MAP3K1, and GAPDH was used as loading control. (b) Values of mean ± S.D. of triplicate experiments were plotted ( ∗ p < 0.05, ∗∗ p < 0.001).

Article Snippet: Reagents included antiglyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (cat. no. KC-5G4; Aksomics, Shanghai, China), antibone morphogenetic protein receptor type 1A (BMPR1A) antibody (cat. no. 12702-1-AP; Proteintech, IL, USA), antimitogen-activated protein kinase kinase kinase 1 (MAP3K1) antibody (cat. no. 19970-1-AP; Proteintech, IL, USA), Magnetic Serum/Plasma DNA Maxi Kit (Tiangen, Beijing, China), TRIzol reagent (Invitrogen, CA, USA), PrimeScript RT kit, and SYBR Premix Ex Taq II (Takara, Shiga, Japan).

Techniques: Expressing, Western Blot, Control

Journal: Journal of cellular and molecular medicine

Article Title: Sodium Hyaluronate-PDGF Repairs Cartilage and Subchondral Bone Microenvironment via HIF-1α-VEGF-Notch and SDF-1-CXCR4 Inhibition in Osteoarthritis.

doi: 10.1111/jcmm.70515

Figure Lengend Snippet: FIGURE 6 | Effects of PDGF-BB and SH-PDGF on NFATc1, CXCR4, ALK1, and ALK5 expression in OA in vivo (5 rats in each group). (A and E) Effects of PDGF-BB and SH-PDGF on NFATc1 expression in subchondral bone determined by IF. (B and F) Effects of PDGF-BB and SH-PDGF on CXCR4 expression in cartilage, as shown by IF. (C and G) Effects of PDGF-BB and SH-PDGF on ALK1 expression in cartilage shown by IF. (D and H) Effects of PDGF-BB and SH-PDGF on ALK5 expression in cartilage, as shown by IF. ns, no significant difference; *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: The following primary antibodies were used: EMCN (1:500, rabbit, Proteintech), CD31 (1:500, rabbit, Proteintech), TRAP (1:200, rabbit, Proteintech), pimonidazole (1:50, mouse, Hypoxyprobe), HIF- 1α (1:500, rabbit, Proteintech), VEGF (1:300, rabbit, Proteintech), Notch1 (1:200, rabbit, Proteintech), Smad2 (1:500, rabbit, Proteintech), p- Smad2 (1:200, rabbit, Proteintech), Smad3 (1:500, rabbit, Proteintech), p- Smad3 (1:200, rabbit, Proteintech), NFATc1 (1:300, rabbit, Proteintech), SDF- 1 (1:300, rabbit, Proteintech), CXCR4 (1:200, rabbit, Proteintech), ALK1 (1:500, rabbit, Proteintech) and ALK5 (1:500, rabbit, Proteintech).

Techniques: Expressing, In Vivo

Journal: iScience

Article Title: DAB2IP suppresses invadopodia formation through destabilizing ALK by interacting with USP10 in breast cancer

doi: 10.1016/j.isci.2023.107606

Figure Lengend Snippet:

Article Snippet: Mouse anti-ALK , Proteintech , Cat# 60321-1-Ig, RRID: AB_2881432.

Techniques: Control, Virus, Recombinant, Magnetic Beads, CCK-8 Assay, Phospho-proteomics, Ab Array, Mutagenesis, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Expressing, CRISPR, Knock-Out, Software